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Zephyr® Molecular Biology Workstation (MBW) - Methods


Below are various method notes using Caliper's Zephyr Molecular Biology Workstation.  Click on the PDF to view the method note or click on the native gwp installer file to add the method to your Zephyr liquid handler.



PCR Setup

 

 

 Method

 Installer to Run Method
  Caliper General Purpose PCR Setup Method PCR Setup Application.gwp
Sequencing Setup
 
 

 Method

 Installer to Run Method

Caliper General Purpose Sequencing Method Sequencing Setup Application.gwp
Normalization
 
 

 Method

 Installer to Run Method
  Caliper General Purpose Normalization Method Plate Normalization Application.gwp
PCR/Sequencing Cleanup
 

 

Method

Installer to Run Method
Automation of the EdgeBio ExcelaPure 96-Well UF PCR Purification Kit on the Zephyr Molecular Biology Workstation (ZH-MN-001) (347 KB) EdgeBio ExcelaPure 96-Well UF PCR Purification Kit.gwp
Automation of the Millipore MultiScreenHTS PCR96 Filter Plate on the Zephyr Molecular Biology Workstation (ZH-MN-003) (352 KB) Millipore MultiScreenHTS PCR 96-Well Plate.gwp
Automation of the Millipore Montage SEQ96 Sequencing Reaction Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-004) (388 KB) Millipore Montage SEQ96 Sequencing Reaction Cleanup Kit.gwp
Automation of the Macherey-Nagel NucleoFast 96 PCR Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-008) (501 KB) Macherey-Nagel NucleoFast 96 PCR.gwp
Automation of the Qiagen QIAquick 96 PCR Purification Kit on the Zephyr Molecular Biology Workstation (ZH-MN-011) (1.0 MB)  
Automation of the Agencourt CleanSEQ Sequencing Reaction Cleanup Kit on the Zephyr Molecular Biology Workstation (ZH-MN-013) (471 KB)  
Automation of the Agencourt Ampure XP SPRI Bead PCR Purification Protocol on the Zephyr Molecular Biology Workstation (ZH-MN-014) (4.4 MB)  
Genomic DNA Isolation/Purification
 

 

 Method

 Installer to Run Method
Automation of the Promega Wizard SV 96 Genomic DNA Purification System on the Zephyr Molecular Biology Workstation (ZH-MN-002) (299 KB) Promega SV 96 Genomic DNA Purification System.gwp
Automation of the Macherey-Nagel NucleoSpin 96 Tissue Kit on the Zephyr Molecular Biology Workstation (ZH-MN-007) (504 KB) Macherey-Nagel NucleoSpin 96 Tissue Kit.gwp
Plasmid DNA Purification
 

 

 Method

 Installer to Run Method
Automation of the Promega Wizard SV 96 Plasmid DNA Purification System on the Zephyr Molecular Biology Workstation (ZH-MN-006) (319 KB) Promega SV 96 Plasmid DNA Purification System.gwp
Automation of the Qiagen QIAprep 96 Turbo Miniprep Kit on the Zephyr Molecular Biology Workstation (ZH-MN-009) (381 KB) QIAprep 96 Turbo Miniprep pDNA.gwp
Automation of the Macherey-Nagel NucleoSpin Robot-96 Plasmid Kit on the Zephyr Molecular Biology Workstation (ZH-MN-012) (1.3 MB)  
RNA Isolation
 

 

 Method

 Installer to Run Method
Automation of the Promega SV 96 Total RNA Isolation System on the Zephyr Molecular Biology Workstation (ZH-MN-005) (308 KB) Promega SV 96 Total RNA Isolation System.gwp
Automation of the Macherey-Nagel NucleoSpin 96 Total RNA Isolation Kit on the Zephyr Molecular Biology Workstation (ZH-MN-010) (492 KB)