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IVIS® Lumina II


The IVIS Lumina II from Caliper Life Sciences provides an expandable, sensitive imaging system that is easy to use for both fluorescent and bioluminescent imaging in vivo. The system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. As the leading optical imaging platform for in vivo analysis, IVIS systems include a range of practical accessories developed through experience in research laboratories worldwide.

Quantitative – Flexible - Expandable
An adjustable field of view from 5 – 12.5 cm and an optional 24cm lens allows imaging of up to 5 mice or 2 medium size rats. The Lumina II can also accommodate Petri dishes or micro-titer plates for in vitro imaging. The system includes premium animal handling features such as a heated stage, gas anesthesia connections and ECG monitoring. High resolution, sharp cut-off filters are simply interchangeable to achieve the highest performance, sensitivity and spectral un-mixing in fluorescence imaging.

Superior Imaging Results
The IVIS Lumina II is capable of imaging both fluorescent and bioluminescent reporters. The system is equipped with up to 21 filter sets that can be used to image reporters that emit from green to near-infrared. Superior spectral un-mixing can be achieved by Lumina II’s optional high resolution short cut off filters. Absolute calibration affords you consistent and reproducible results independent of magnification, filter selection from one instrument to any another IVIS instrument within an organization or around the world. The Living Image software yields high-quality, reproducible, quantitative results incorporating instrument calibration, background subtraction and the image algorithms.

For more information in our IVIS® Lumina II imaging system please click the button below and complete our product & services inquiry form. 

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or contact David.Panzarella@perkinelmer.com




Applications

• Oncology Research (PDF)
• Infectious Disease
• Inflammation
• Metabolic Diseases
• Neurology 
• Gene Therapy
• Stem cell Biology
• Cardiovascular Disease
• Immunology & Transplantation Biology
• Toxicology (PDF)
• Drug Metabolism Studies

Features

• High-sensitivity in vivo and in vitro imaging of fluorescence and bioluminescence
• Adjustable field of view from 5 - 12.5 cm (for up to 3 mice or a medium sized rat)
• Camera CCD provides high quantum efficiency over the entire visible to near-infrared spectrum and is thermo-electrically (Peltier) cooled to -90 C to ensure low dark current and low noise.
• Light-tight imaging chamber
• 8 position emission filter wheel and 12 position excitation filter wheel
     - A set of four filter pairs for fluorescent imaging come standard with the instrument, in addition to a set of four background filters for subtraction of tissue autofluorescence.
• Heated stage to maintain optimum body temperature
• Motor controlled stage, filter wheel, lens position, and f-stop
• NIST traceable absolute calibrations
• Gas anesthesia inlet and outlet ports

Demos

Our product can be demonstrated at our facility in Hopkinton, Massachussetts or other Caliper Life Sciences offices worldwide.  Please contact your local Caliper Representative to arrange a demo.

Software

Living Image Software 1 acquisition copy and 4 analysis copies of Living Image software 3.0.4x and higher

Specifications

Power Requirements

6 Amps at 120V

Dimensions

Imaging Chamber Interior Size: 43 x 38 x 43 cm (W x D x H)
Imaging System Space Requirement: 48 x 71 x 104 cm (W x D x H)

Operating System Software

Living Image Software 1 acquisition copy and 4 analysis copies of Living Image software 3.0.4x and higher

Camera Sensor

Back-thinned, back-illuminated Grade 1 CCD

CCD

Size 1.33 x 1.33 cm
Operating Temperature -90°C

Imaging Pixels

1024 x 1024

Quantum Efficiency

>85% 500 – 700 nm
>30% 400 – 900nm

Pixel Size

13 micron square

Minimum Detectable Radiance

100 photons/s/sr/cm2 (bin = 16, 5 min. exposure, f/1)

Field of View (FOV)

Minimum FOV 5 x 5 cm
Maximum FOV 12.5 x 12.5 cm (optional 23 x 24 cm)

Minimum Image Pixel Resolution

50 microns

Read Noise

< 3 electrons for bin=1,2,4
< 5 electrons for bin=8,16

Dark Current (Typical)

<120 electrons/s/cm2 or 2 x 10-4 electrons/s/pixel

Fluorescence Filters

Excitation 10 high resolution filters standard on all instruments
Emission 5 sets available to choose from: One standard resolution set of 4 filters and 4 sets of 7 high resolution filters

Fluorescence Bkg. Subtraction Filters

Included

Computer

Minimum specification - 2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen monitor

Lens

f/0.95 – f/16, 50 mm

Stage Temperature

20 – 40°C