XenoLight RediJect 2-DG-750 Probe
XenoLight RediJect 2-DeoxyGlucosone (DG) 750 is a fluorescent probe for in vivo targeting of tumors that typically exhibit elevated glucose uptake rate in comparison to surrounding tissues. To achieve maximum glucose targeting and enhanced tumor uptake, the probe has been designed to contain four 2-DG molecules per dye molecule. As indicated in the figure 1, RediJect 2-DG 750 probe showed specific accumulation to LL-2 tumors shortly after delivery. In addition, specific tumor labeling with the RediJect 2-DG 750 probe has also been demonstrated with Prostate (PC-3M) and Lung (NCI-H460) tumors, further validating the utility of this probe as a generic fluorescence reagent for in vivo tumor targeting.
• Novel ready-to-use probe to monitor glucose uptake in vivo non-invasively• Dispensed to image 5 animals (explorer kit) or 20 animals (standard kit)
• Maximum glucose targeting and enhanced tumor uptake
• In vivo imaging quality, validated on IVIS imaging systems
XenoLight RediJect 2-DG-750 Probe (Explorer kit)
XenoLight RediJect 2-DG-750 Probe (Standard kit)
In vivo detection of Glucose uptake with the 2DG-750 probe
C57 BL/6 albino mice with s.c. with 1x106 LL2-luc tumor cells. At 2 weeks, tumors were approximately 200 mm3. Mice were intravenously injected with 10 nmol of 2-DG-750 probe or control IF750 dye and imaged at 3, 7, 24 and 72 hours with IVIS Spectrum (Ex745nm/Em820nm ). Mice injected with the 2-DG-750 probe showed specific accumulation of the probe to the tumor sites. Quantification showed a peak signal at 3 hours and the tumor remained labeled at 72 hours. In comparison, mice that were injected with 750 control dye showed no specific targeting at the tumor site at 3 hours. Bioluminescence imaging showed similar luciferase activity in all the mice correlating with tumor volume.
Features
Concentration: 10nmoles/100 ul
Shipping condition: The kit will be shipped in cold gel packs to avoid temperature variations
Volume per vial: Explorer kit:1 sterile amber vial containing 600ul of 10nmoles/100ul probe
Standard kit: 4 sterile amber vials containing 600ul of 10nmoles/100ul probe
Storage and Handling: Store the 2-DG 750 probe at 4°C and protect from light. For in vivo imaging studies we recommend an intravenous injection of 100 ul/mouse. Allow the probe to warm up to room temperature before injection in an animal
Dye Characteristics:
Peak Excitation: 750 nm, Peak Emission: 780 nm
Ideal IVIS Spectrum Filter Sets: Ex 745 nm/Em 820 nm
Alternatively you can also use the spectral unmixing filter sets using imaging wizard program in the Living Image software.