Microfluidic Publications

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2011 2010 2009 2008 2007 2006 2005 2004 2002

2012

Activation of aurora-a kinase by protein partner binding and phosphorylation are independent and synergistic

2011

A comparative study of fragment screening methods on the p38alpha kinase: new methods, new insights

A high-throughput microchip-based glycan screening assay for antibody cell culture samples

A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Assessing protein-RNA interactions using microfluidic capillary mobility shift assays

AST1306, a novel irreversible inhibitor of the epidermal growth factor receptor 1 and 2, exhibits antitumor activity both in vitro and in vivo

Decontamination of MDA reagents for single cell whole genome amplification

Design and evaluation of 3-aminopyrazolopyridinone kinase inhibitors inspired by the natural product indirubin

Genotyping of Saccharomyces cerevisiae strains by interdelta sequence typing using automated microfluidics

High throughput MLVA-16 typing for Brucella based on the microfluidics technology

High throughput profiling of charge heterogeneity in antibodies by microchip electrophoresis

New ligation-independent cloning vectors compatible with a high-throughput platform for parallel construct expression evaluation using baculovirus-infected insect cells

Novel library of selenocompounds as kinase modulators

Prospective Genomic Characterization of the German Enterohemorrhagic Escherichia coli O104:H4 Outbreak by Rapid Next Generation Sequencing Technology

Study of the PDK1/AKT signaling pathway using selective PDK1 inhibitors, HCS, and enhanced biochemical assays

Use of fluorescence lifetime technology to provide efficient protection from false hits in screening applications

Virtual screening and further development of novel ALK inhibitors

2010

A 454 multiplex sequencing method for rapid and reliable genotyping of highly polymorphic genes in large-scale studies

A silent mutation made possible efficient production of active human Frk tyrosine kinase in Escherichia coli

Accessing protein methyltransferase and demethylase enzymology using microfluidic capillary electrophoresis

Alternative splicing enriched cDNA libraries identify breast cancer-associated transcripts

An electrophoretic mobility shift assay for the identification and kinetic analysis of acetyl transferase inhibitors

Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690,550, in rat adjuvant-induced arthritis

Capillary microfluidic electrophoretic mobility shift assays: application to enzymatic assays in drug discovery

Combined use of fluorescent dyes and flow cytometry to quantify the physiological state of Pichia pastoris during the production of heterologous proteins in high-cell-density fed-batch cultures

Crystal structure of an Aurora-A mutant that mimics Aurora-B bound to MLN8054: insights into selectivity and drug design

Development of a capillary gel electrophoresis method for monitoring disulfide isomer heterogeneity in IgG2 antibodies

Development, validation, and implementation of capillary gel electrophoresis as a replacement for SDS-PAGE for purity analysis of IgG2 mAbs

Differential effects of divalent manganese and magnesium on the kinase activity of leucine-rich repeat kinase 2 (LRRK2)

Discovery of an orally efficacious imidazo[5, 1-f][1,2,4]triazine Dual inhibitor of IGF-1R and IR

Epigenetics: Tools and Technologies

Expression, purification, characterization and crystallization of non- and phosphorylated states of JAK2 and JAK3 kinase domain

Extended kinase profile and properties of the protein kinase inhibitor nilotinib

Fragment-based screening by biochemical assays: Systematic feasibility studies with trypsin and MMP12

Novel synthesis and structural characterization of a high-affinity paramagnetic kinase probe for the identification of non-ATP site binders by nuclear magnetic resonance

Protein lysine methyltransferase G9a inhibitors: design, synthesis, and structure activity relationships of 2,4-diamino-7-aminoalkoxy-quinazolines

Stability of IgG isotypes in serum

Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex

The small molecule specific EphB4 kinase inhibitor NVP-BHG712 inhibits VEGF driven angiogenesis

Widening the bottleneck: increasing success in protein expression and purification

2009

A high throughput dimer screening assay for monoclonal antibodies using chemical cross-linking and microchip electrophoresis

A novel, highly selective, tight binding IkappaB kinase-2 (IKK-2) inhibitor: a tool to correlate IKK-2 activity to the fate and functions of the components of the nuclear factor-kappaB pathway in arthritis-relevant cells and animal models

A predictive high-throughput scale-down model of monoclonal antibody production in CHO cells

Affinity classification of kinase inhibitors by mass spectrometric methods and validation using standard IC(50) measurements

Designing experiments for high-throughput protein expression

Discovery of novel sphingosine kinase 1 inhibitors

Discovery of OSI-906: a selective and orally efficacious dual inhibitor of the IGF-1 receptor and insulin receptor

E. coli and insect cell expression, automated purification and quantitative analysis

High throughput microchip-based separation and quantitation of high-molecular-weight glutenin subunits

High-throughput biochemical kinase selectivity assays: panel development and screening applications

High-throughput screening of the cyclic AMP-dependent protein kinase (PKA) using the caliper microfluidic platform

Identification and preclinical characterization of AZ-23, a novel, selective, and orally bioavailable inhibitor of the Trk kinase pathway

Identification of allosteric PIF-pocket ligands for PDK1 using NMR-based fragment screening and 1H-15N TROSY experiments

Identification of death-associated protein kinases inhibitors using structure-based virtual screening

Kinetic mechanism and inhibitor characterization of WNK1 kinase

Measuring and interpreting the selectivity of protein kinase inhibitors

Methods for generating shotgun and mixed shotgun/paired-end libraries for the 454 DNA sequencer

MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis

Mocr: a novel fusion tag for enhancing solubility that is compatible with structural biology applications

Multiplex assay for simultaneously typing and subtyping influenza viruses by use of an electronic microarray

PD-1 blockade in rhesus macaques: impact on chronic infection and prophylactic vaccination

The effect of Fc glycan forms on human IgG2 antibody clearance in humans

2008

Controlling data quality and reproducibility of a high-sensitivity immunoassay using isotachophoresis in a microchip

Effects of the JAK2 inhibitor, AZ960, on Pim/BAD/BCL-xL survival signaling in the human JAK2 V617F cell line SET-2

High-throughput assays for sirtuin enzymes: a microfluidic mobility shift assay and a bioluminescence assay

Human IgG2 antibody disulfide rearrangement in vivo

Microchip assays for screening monoclonal antibody product quality

Multiple and specific mRNA processing targets for the major human hnRNP proteins

Nucleic acid amplification of individual molecules in a microfluidic device

Predicting kinase selectivity profiles using Free-Wilson QSAR analysis

Use of MLVA-16 typing to trace the source of a laboratory-acquired Brucella infection

Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex

2007

A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis

Development of a protease production platform for structure-based drug design

Enhanced bacterial protein expression during auto-induction obtained by alteration of lac repressor dosage and medium composition

2006

A high-throughput microfluidic assay for SH2 domain-containing inositol 5-phosphatase 2

A microfluidics-based mobility shift assay to discover new tyrosine phosphatase inhibitors

Assay development and screening of a serine/threonine kinase in an on-chip mode using caliper nanofluidics technology

Overcoming the hurdle of fluorescent compounds in kinase screening: a case study

Screening factors effecting a response in soluble protein expression: formalized approach using design of experiments

2005

Agonist-induced calcium response in single human platelets assayed in a microfluidic device

High-throughput mouse genotyping using robotics automation

Mitochondrial DNA as a cancer biomarker

2004

Comparison of on-chip and off-chip microfluidic kinase assay formats

Functional protein microarrays: ripe for discovery

Two simple and generic antibody-independent kinase assays: comparison of a bioluminescent and a microfluidic assay format

2003

Assay development and high-throughput screening of caspases in microfluidic format

Separation of phospholipids in microfluidic chip device: application to high-throughput screening assays for lipid-modifying enzymes